A simple non-invasive method for measuring gross brain size in small live fish with semi-transparent heads
Author(s) -
Joacim Näslund
Publication year - 2014
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.586
Subject(s) - zebrafish , optic tectum , measure (data warehouse) , fish <actinopterygii> , brain size , tectum , trout , biology , computer science , fishery , neuroscience , medicine , central nervous system , radiology , biochemistry , database , midbrain , gene , magnetic resonance imaging
This paper describes a non-invasive method for estimating gross brain size in small fish with semi-transparent heads, using system camera equipment. Macro-photographs were taken from above on backlit free-swimming fish undergoing light anaesthesia. From the photographs, the width of the optic tectum was measured. This measure (TeO-measure) correlates well with the width of the optic tectum as measured from out-dissected brains in both brown trout fry and zebrafish (Pearson r > 0.90). The TeO-measure also correlates well with overall brain wet weight in brown trout fry ( r = 0.90), but less well for zebrafish ( r = 0.79). A non-invasive measure makes it possible to quickly assess brain size from a large number of individuals, as well as repeatedly measuring brain size of live individuals allowing calculation of brain growth.
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