The mitochondrial genomes of two walnut pests,Gastrolina depressa depressaandG.depressa thoracica(Coleoptera: Chrysomelidae), and phylogenetic analyses

In this study, the mitochondrial genomes (mitogenomes) of two walnut leaf insect pests, Gastrolina depressa depressa and G . depressa thoracica , were sequenced by Sanger sequencing technology. The mitogenome of G . depressa thoracica was complete at 16,109 bp in length, while the mitogenome of G . depressa depressa (14,277 bp) was partial. The genomic analyses indicated that both mitogenomes have the typical gene content and arrangement. The formerly identified elements, ‘TAGTA’ between trnSer ( UCN ) and nad2 , and ‘ATGATAA’ between atp8 and atp6 , were more conserved than that between nad4L and nad4 , which was ‘ATGTTAA’ in Coleoptera excluding Polyphaga. Phylogenetic analyses of the 13 protein-coding genes from 36 coleopteran species well supported a close affinity between the subfamily Chrysomelinae including G . depressa thoracica and G . depressa depressa and Galerucinae, as well as a sister relationship of ((Eumolpinae + Cryptocephalinae) + Cassidinae) within Chrysomelidae.