Open AccessEstablishment and quality evaluation of a glioma biobank in Beijing Tiantan Hospital
Author(s) -
Fanhong Kong,
Wenli Zhang,
Lin Qiao,
Qi Li,
Haowen Li,
Jingli Cao,
Wenyan He,
Chengya Dong,
Yanjiao He,
Lu He,
Li Liu,
Weilun Fu,
Lijun Liu,
Zirui Li,
Yajie Wang
Publication year - 2018
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.4450
Subject(s) - biobank , housekeeping gene , beijing , medicine , biology , bioinformatics , gene , geography , gene expression , genetics , archaeology , china
Background We established a glioma biobank at Beijing Tiantan Hospital in November, 2010. Specialized residents have been trained to collect, store and manage the biobank in accordance with standard operating procedures. Methods One hundred samples were selected to evaluate the quality of glioma samples stored in the liquid nitrogen tank during different periods (from 2011 to 2015) by morphological examination, RNA integrity determination, DNA integrity determination and housekeeping gene expression determination. Results The majority of samples (95%) had high RNA quality for further analysis with RIN ≥6. Quality of DNA of all samples were stable without significant degradation. Conclusion Storage conditions of our biobank are suitable for long-term (at least five years) sample preservation with high molecular quality.
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