English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Pantoea stewartii  subsp.  stewartii  is a Gram-negative proteobacterium that causes leaf blight and Stewart’s wilt disease in corn. Quorum sensing (QS) controls bacterial exopolysaccharide production that blocks water transport in the plant xylem at high bacterial densities during the later stage of the infection, resulting in wilt. At low cell density the key master QS regulator in  P. stewartii , EsaR, directly represses  rcsA , encoding an activator of capsule biosynthesis genes, but activates  lrhA , encoding a transcription factor that regulates surface motility. Both RcsA and LrhA have been shown to play a role in plant virulence. In this study, additional information about the downstream targets of LrhA and its interaction with RcsA was determined. A transcriptional fusion assay revealed autorepression of LrhA in  P. stewartii  and electrophoretic mobility shift assays (EMSA) using purified LrhA confirmed that LrhA binds to its own promoter. In addition, LrhA binds to the promoter for the RcsA gene, as well as those for putative fimbrial subunits and biosurfactant production enzymes in  P. stewartii,  but not to the  flhDC  promoter, which is the main direct target of LrhA in   Escherichia coli.  This work led to a reexamination of the physiological function of RcsA in  P. stewartii  and the discovery that it also plays a role in surface motility. These findings are broadening our understanding of the coordinated regulatory cascades utilized in the phytopathogen  P. stewartii .

peerj

Integrated downstream regulation by the quorum-sensing controlled transcription factors LrhA and RcsA impacts phenotypic outputs associated with virulence in the phytopathogenPantoea stewartiisubsp.stewartii