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Background  Pesticidal RNAs that silence critical gene function have great potential in pest management, but the benefits of this technology must be weighed against non-target organism risks.    Methods  Published studies that developed pesticidal double stranded RNAs (dsRNAs) were collated into a database. The target gene sequences for these pesticidal RNAs were determined, and the degree of similarity with sequences in the honey bee genome were evaluated statistically.    Results  We identified 101 insecticidal RNAs sharing high sequence similarity with genomic regions in honey bees. The likelihood that off-target sequences were similar increased with the number of nucleotides in the dsRNA molecule. The similarities of non-target genes to the pesticidal RNA was unaffected by taxonomic relatedness of the target insect to honey bees, contrary to previous assertions. Gene groups active during honey bee development had disproportionately high sequence similarity with pesticidal RNAs relative to other areas of the genome.    Discussion  Although sequence similarity does not itself guarantee a significant phenotypic effect in honey bees by the primary dsRNA,  in silico  screening may help to identify appropriate experimental endpoints within a risk assessment framework for pesticidal RNAi.

In silico identification of off-target pesticidal dsRNA binding in honey bees (Apis mellifera)