A simple electroelution method for rapid protein purification: isolation and antibody production of alpha toxin fromClostridium septicum | Zendy

Lorena VázquezIglesias | Zendy; Borja Estefanell-Ucha | Zendy; Leticia Barcia-Castro | Zendy; Marı́a Páez de la Cadena | Zendy; Paula Álvarez-Chaver | Zendy; Daniel Ayude-Vázquez | Zendy; Francisco Javier Rodrı́guez-Berrocal | Zendy

Open Access

A simple electroelution method for rapid protein purification: isolation and antibody production of alpha toxin fromClostridium septicum

Author(s) -

Lorena VázquezIglesias,

Borja Estefanell-Ucha,

Leticia Barcia-Castro,

Marı́a Páez de la Cadena,

Paula Álvarez-Chaver,

Daniel Ayude-Vázquez,

Francisco Javier Rodrı́guez-Berrocal

Publication year - 2017

Publication title -

peerj

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.927

H-Index - 70

ISSN - 2167-8359

DOI - 10.7717/peerj.3407

Subject(s) - clostridium septicum , toxin , western blot , biology , microbiology and biotechnology , electroelution , antibody , antiserum , polyclonal antibodies , clostridium perfringens , clostridium , biochemistry , bacteria , polyacrylamide gel electrophoresis , enzyme , immunology , genetics , gene

Clostridium septicum produces a number of diseases in human and farm animals which, in most of the cases, are fatal without clinical intervention. Alpha toxin is an important agent and the unique lethal virulent factor produced by Clostridium septicum. This toxin is haemolytic, highly lethal and necrotizing activities but is being used as an antigen to develop animal vaccines. The aim of this study was to isolate the alpha toxin of Clostridium septicum and produce highly specific antibodies against it. In this work, we have developed a simple and efficient method for alpha toxin purification, based on electroelution that can be used as a time-saving method for purifying proteins. This technique avoids contamination by other proteins that could appear during other protein purification techniques such chromatography. The highly purified toxin was used to produce polyclonal antibodies. The specificity of the antibodies was tested by western blot and these antibodies can be applied to the quantitative determination of alpha toxin by slot blot.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research