Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology | Zendy

Natália J. Mendes | Zendy; Vanessa P. Cruz | Zendy; Fernando Yuldi Ashikaga | Zendy; Sâmia M. Camargo | Zendy; Cláudio Oliveira | Zendy; Andrew N. Piercy | Zendy; George H. Burgess | Zendy; Rui Coelho | Zendy; Miguel N. Santos | Zendy; Fernando Fernandes Mendonça | Zendy; Fausto Foresti | Zendy

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Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology

Author(s) -

Natália J. Mendes,

Vanessa P. Cruz,

Fernando Yuldi Ashikaga,

Sâmia M. Camargo,

Cláudio Oliveira,

Andrew N. Piercy,

George H. Burgess,

Rui Coelho,

Miguel N. Santos,

Fernando Fernandes Mendonça,

Fausto Foresti

Publication year - 2016

Publication title -

peerj

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.927

H-Index - 70

ISSN - 2167-8359

DOI - 10.7717/peerj.2205

Subject(s) - biology , pyrosequencing , threatened species , iucn red list , microsatellite , conservation genetics , tiger , genetic diversity , fishery , near threatened species , carcharhinus , population , zoology , evolutionary biology , ecology , allele , genetics , habitat , demography , computer security , gene , computer science , sociology

The tiger shark ( Galeocerdo cuvier ) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus . Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.

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