Assessing Illumina technology for the high-throughput sequencing of bacteriophage genomes | Zendy

Branko Rihtman | Zendy; Sean Meaden | Zendy; Martha R. J. Clokie | Zendy; Britt Koskella | Zendy; Andrew Millard | Zendy

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Assessing Illumina technology for the high-throughput sequencing of bacteriophage genomes

Author(s) -

Branko Rihtman,

Sean Meaden,

Martha R. J. Clokie,

Britt Koskella,

Andrew Millard

Publication year - 2016

Publication title -

peerj

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.927

H-Index - 70

ISSN - 2167-8359

DOI - 10.7717/peerj.2055

Subject(s) - genome , biology , in silico , bacteriophage , computational biology , dna sequencing , bacterial genome size , sequence assembly , genetics , illumina dye sequencing , whole genome sequencing , genomics , gene , escherichia coli , transcriptome , gene expression

Bacteriophages are the most abundant biological entities on the planet, playing crucial roles in the shaping of bacterial populations. Phages have smaller genomes than their bacterial hosts, yet there are currently fewer fully sequenced phage than bacterial genomes. We assessed the suitability of Illumina technology for high-throughput sequencing and subsequent assembly of phage genomes. In silico datasets reveal that 30× coverage is sufficient to correctly assemble the complete genome of ~98.5% of known phages, with experimental data confirming that the majority of phage genomes can be assembled at 30× coverage. Furthermore, in silico data demonstrate it is possible to co-sequence multiple phages from different hosts, without introducing assembly errors.

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