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Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacle  Chelonibia testudinaria  ( Linnaeus, 1758 ).  Chelonibia testudinaria  appears to be a host generalist, and has an unusual sexual system, androdioecy. Genetic studies on host specificity and mating behavior are impeded by the lack of fine-scale, highly variable markers, such as microsatellite markers. In the present study, we discovered thousands of new microsatellite loci from next-generation sequencing data, and characterized 12 loci thoroughly. We conclude that 11 of these loci will be useful markers in future ecological and evolutionary studies on  C. testudinaria .

peerj

Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle<i>Chelonibia testudinaria</i>(Linnaeus, 1758)

Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacleChelonibia testudinaria(Linnaeus, 1758)