Development and characterization of 26 novel microsatellite loci for the trochid gastropodGibbula divaricata(Linnaeus, 1758), using IlluminaMiSeqnext generation sequencing technology | Zendy

Violeta LópezMárquez | Zendy; Ricardo García-Jiménez | Zendy; José Templado | Zendy; Annie Machordom | Zendy

Open Access

Development and characterization of 26 novel microsatellite loci for the trochid gastropodGibbula divaricata(Linnaeus, 1758), using IlluminaMiSeqnext generation sequencing technology

Author(s) -

Violeta LópezMárquez,

Ricardo García-Jiménez,

José Templado,

Annie Machordom

Publication year - 2016

Publication title -

peerj

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.927

H-Index - 70

ISSN - 2167-8359

DOI - 10.7717/peerj.1789

Subject(s) - biology , microsatellite , linkage disequilibrium , locus (genetics) , illumina dye sequencing , sympatric speciation , dna sequencing , evolutionary biology , genetics , allele , haplotype , gene

In the present study we used the high-throughput sequencing technology Illumina MiSeq to develop 26 polymorphic microsatellite loci for the marine snail Gibbula divaricata . Four to 32 alleles were detected per locus across 30 samples analyzed. Observed and expected heterozygosities ranged from 0.130 to 0.933 and from 0.294 to 0.956, respectively. No significant linkage disequilibrium existed. Seven loci deviated from Hardy-Weinberg equilibrium that could not totally be explained by the presence of null alleles. Sympatric distribution with other species of the genus Gibbula , as G. rarilineata and G. varia , lead us to test the cross utility of the developed markers in these two species, which could be useful to test common biogeographic patterns or potential hybridization phenomena, since morphological intermediate specimens were found.

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