Open AccessProtective effects of the extracts ofBarringtonia racemosashoots against oxidative damage in HepG2 cells
Author(s) -
Kin Weng Kong,
Sarni Mat Junit,
Norhaniza Aminudin,
Fouad Abdulrahman Hassan,
Amin Ismail,
Azlina Abdul Aziz
Publication year - 2016
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.1628
Subject(s) - ellagic acid , oxidative stress , superoxide dismutase , kaempferol , gallic acid , reactive oxygen species , catalase , quercetin , chemistry , lipid peroxidation , protocatechuic acid , antioxidant , biochemistry , cytotoxicity , viability assay , traditional medicine , polyphenol , biology , cell , medicine , in vitro
Barringtonia racemosa is a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H 2 O 2 -induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage.
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