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In this study, we sequenced the genome of  Pandoraea pnomenusa  RB38 using Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. A pair of cognate  luxI/R  homologs was identified where the  luxI  homolog,  ppnI,  was found adjacent to a  luxR  homolog,  ppnR1 . An additional orphan  luxR  homolog,  ppnR 2, was also discovered. Multiple sequence alignment and phylogenetic analysis revealed that  ppnI  is an  N -acyl homoserine lactone (AHL) synthase gene that is distinct from those of the nearest phylogenetic neighbor  viz. Burkholderia  spp. High resolution tandem mass spectrometry (LC-MS/MS) analysis showed that  Escherichia coli  BL21 harboring  ppnI  produced a similar AHL profile ( N -octanoylhomoserine lactone, C8-HSL) as  P. pnomenusa  RB38, the wild-type donor strain, confirming that PpnI directed the synthesis of AHL in  P. pnomenusa  RB38. To our knowledge, this is the first documentation of the  luxI/R  homologs of the genus  Pandoraea .

Complete genome sequencing ofPandoraea pnomenusaRB38 and Molecular Characterization of ItsN-acyl homoserine lactone synthase geneppnI