English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The fungus  Colletotrichum cereale  incites anthracnose disease on  Poa annua  (annual bluegrass) turfgrass. Anthracnose disease is geographically widespread throughout the world and highly destructive to cool-season turfgrasses, with infections by  C. cereale  resulting in extensive turf loss. Comprehensive research aimed at controlling turfgrass anthracnose has been performed in the field, but knowledge of the causal organism and its basic biology is still needed. In particular, the lack of a reliable greenhouse-based inoculation protocol performed under controlled environmental conditions is an obstacle to the study of  C. cereale  and anthracnose disease. Our objective was to develop a consistent and reproducible inoculation protocol for the two major genetic lineages of  C. cereale . By adapting previously successful field-based protocols and combining with components of existing inoculation procedures, the method we developed consistently produced  C. cereale  infection on two susceptible  P. annua  biotypes. Approximately 7 to 10 days post-inoculation, plants exhibited chlorosis and thinning consistent with anthracnose disease symptomology. Morphological inspection of inoculated plants revealed visual signs of the fungus (appressoria and acervuli), although acervuli were not always present. After stringent surface sterilization of inoculated host tissue,  C. cereale  was consistently re-isolated from symptomatic tissue. Real-time PCR detection analysis based on the  Apn2  marker confirmed the presence of the pathogen in host tissue, with both lineages of  C. cereale  detected from all inoculated plants. When a humidifier was not used, no infection developed for any biotypes or fungal isolates tested. The inoculation protocol described here marks significant progress for  in planta  studies of  C. cereale , and will enable scientifically reproducible investigations of the biology, infectivity and lifestyle of this important grass pathogen.

Development of a greenhouse-based inoculation protocol for the fungusColletotrichum cerealepathogenic to annual bluegrass (Poa annua)