Looking at microbial metabolism by high-resolution2H-NMR spectroscopy | Zendy

V. P. Kutyshenko | Zendy; Petr M. Beskaravayny | Zendy; Maxim Molchanov | Zendy; S. I. Paskevich | Zendy; Dmitry A. Prokhorov | Zendy; Vladimir N. Uversky | Zendy

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Looking at microbial metabolism by high-resolution²H-NMR spectroscopy

Author(s) -

V. P. Kutyshenko,

Petr M. Beskaravayny,

Maxim Molchanov,

S. I. Paskevich,

Dmitry A. Prokhorov,

Vladimir N. Uversky

Publication year - 2013

Publication title -

peerj

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.927

H-Index - 70

ISSN - 2167-8359

DOI - 10.7717/peerj.101

Subject(s) - metabolism , nuclear magnetic resonance spectroscopy , high resolution , biochemistry , cellular metabolism , chemistry , aqueous solution , cell metabolism , mitochondrion , biology , stereochemistry , organic chemistry , remote sensing , geology

We analyzed the applicability of high-resolution 2 H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D 2 O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.

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