Open AccessAssessment of phytochemicals and antioxidant activities of raw and germinating Ceiba pentandra (kapok) seeds
Author(s) -
Chekuboyina Ravi Kiran,
Dadi Bhaskara Rao,
Sirisha Nagala,
T. Raghava Rao
Publication year - 2015
Publication title -
journal of biomedical research/journal of biomedical research
Language(s) - English
Resource type - Journals
eISSN - 2352-4685
pISSN - 1674-8301
DOI - 10.7555/jbr.27.20120145
Subject(s) - phytochemical , ceiba , antioxidant , chemistry , germination , radical , reactive oxygen species , dpph , oxygen radical absorbance capacity , botany , food science , traditional medicine , biochemistry , biology , medicine , ecology
To determine the significance of germination on phytochemical constituents and non-enzymatic antioxidant activities of Ceiba pentandra seed extracts. Phytochemicals and antioxidant activities of raw and germinating seeds of Ceiba pentandra were estimated by different methods. The levels of phytochemical constituents were influenced by germination and increased except alkaloids and tannins, which were decreased significantly during germination. Among non-enzymatic antioxidants like DPPH, FRAP, reducing assay and hydroxyl radical scavenging activity all showed improved activity compared with non-germinating seeds. This may be due to various reactive oxygen species (ROS) that were generated as by-products of metabolism during germination. This group of ROS included superoxide radicals (O2), hydrogen peroxide radicals (H2O2) and hydroxyl radicals (OH). The formation of these oxygen radicals resulted in the accumulation of lipid hydroperoxides by radical chain oxidation via phospholipids peroxy radicals within membranes. Therefore, it was hypothesized that this could be related to the increase of antioxidant activity in large unilamellar vesicles observed in germinated seeds. The implication of this study is that the Ceiba pentandra seeds as natural antioxidant agents and put forward the possibility of employing for therapeutic potential.