Open AccessThermal Inactivation and Aggregation of Lysozyme in the Presence of Nano- TiO2 and Nano-SiO2 in Neutral pH
Author(s) -
Behzad Shareghi,
A R Hashemian,
Sadegh Farhadian,
Masoud SalavatiNiasari,
Behnaz Saffar,
Hamdollah Moshtaghi
Publication year - 2013
Publication title -
shilap revista de lepidopterología
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.338
H-Index - 12
eISSN - 2340-4078
pISSN - 0300-5267
DOI - 10.7508/jns.2013.03.003
Subject(s) - nano , materials science , lysozyme , thermal , nanotechnology , chemical engineering , composite material , biochemistry , chemistry , thermodynamics , physics , engineering
Protein aggregation is a problem in biotechnology. High temperature is one of the most important reasons to enhance enzyme inactivation and aggregation in industrial systems. This work focuses on the effect of TiO2 and SiO2 nanoparticles on refolding and reactivation of lysozyme. In the presence of TiO2 and SiO2 nanoparticles, after enzyme heat treatment at 98◦C for 30 min, not only aggregates were observed, but the amount of those increased. Hence the residual activity of lysozyme (without additives) and even in the presence of TiO2 and SiO2 nanoparticles after heat treatment was very low
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