A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR QUANTIFICATION OF TRANDOLAPRIL USING UV SPECTROMETRIC DETECTION | Zendy

Sunil Kumar Dubey | Zendy; Swapnil Deshpande | Zendy; Sandeep Kumar | Zendy; Prashant P. Raut | Zendy; Akash Kumar Jain | Zendy; Rajeev J. Mudakavi | Zendy

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A HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR QUANTIFICATION OF TRANDOLAPRIL USING UV SPECTROMETRIC DETECTION

Author(s) -

Sunil Kumar Dubey,

Swapnil Deshpande,

Sandeep Kumar,

Prashant P. Raut,

Akash Kumar Jain,

Rajeev J. Mudakavi

Publication year - 2011

Publication title -

international journal of biomedical research

Language(s) - English

Resource type - Journals

eISSN - 2455-0566

pISSN - 0976-9633

DOI - 10.7439/ijbr.v1i3.66

Subject(s) - trandolapril , chromatography , detection limit , chromatographic separation , acetonitrile , high performance liquid chromatography , medicine , analytical chemistry (journal) , chemistry , blood pressure , angiotensin converting enzyme , ace inhibitor

A high performance Liquid chromatographic method for quantification of trandolapril using UV detection was developed and validated. Trandolapril samples were analysed on Merck LiChroCART -RP C18 column (250x4.0, i.d.5 µm) and the mobile phase composition used for detection was combination of acetonitrile: methanol: phosphate buffer (0.025mM) pH3.0 (40:35:25)at a flow rate of 1 ml/min. The λmax used was 220nm with UV detection. The retention time of trandolapril by proposed method was found to be 2.750 ± 0.008 min. Peak area obtained were linearly related to concentration of drug in samples in range of 2.5-17.5 µg/mL having correlation coefficient of 0.999. The LOD and LOQ of trandolapril by proposed method was found to be 0.099 µg/mL and 0.300834 µg/ml respectively. The method was validated as per ICH guidelines for various parameters. The results for accuracy, precision and robustness were found to be within accepted limits.

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