Sanitary Status of Pome and Stone Fruit Collection in Gene Bank in Republic of Srpska
Author(s) -
Gordana Đurić,
Biljana Lolić,
Mirela Kajkut Zeljković,
D. Delić,
Mirjana Koprivica,
Mariana Radulović,
Petar Nikolić,
Nikola Mićić,
Živojin Erić
Publication year - 2016
Publication title -
агрознање
Language(s) - English
Resource type - Journals
eISSN - 2233-0070
pISSN - 1512-6412
DOI - 10.7251/agren1501121d
Subject(s) - pome , biology , pear , horticulture , pox virus , prunus , virus , rosaceae , botany , virology
Detection of viruses presence were carried out for the 225 a of pome and stone fruit trees from the collection of the Genetic Resources Institute of University of Banja Luka, located within the Botanical Garden of the University, tested by DAS-ELISA. The pome fruit trees were analyzed on presence of the following viruses: Apple Chlorotic Leaf Spot Virus (ACLSV), Apple Stem Grooving Virus (ASGV), Apple Stem Pitting Virus (ASPV) and Apple Mosaic Virus (ApMV). The stone fruits were analyzed on presence of Plum Pox Virus (PPV), Prune Dwarf Virus (PDV) and Prunus Necrotic Ring Spot Virus (PNRSV). All samples were tested serologically by DAS-ELISA. In addition to this, virus negative pear and apple accessions were tested for 'Candidatus Phytoplasma mali' and 'Candidatus Phytoplasma pyri' presence using nested-PCR/RFLP analyses.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom