Open AccessAmmonium sulfate precipitation combined with liquid chromatography is sufficient for purification of bovine serum albumin that is suitable for most routine laboratory applications
Author(s) -
Odutayo O. Odunuga,
Alina Shazhko
Publication year - 2013
Publication title -
biochemical compounds
Language(s) - English
Resource type - Journals
ISSN - 2052-9341
DOI - 10.7243/2052-9341-1-3
Subject(s) - bovine serum albumin , chromatography , ammonium sulfate , precipitation , ammonium sulfate precipitation , protein precipitation , chemistry , albumin , sulfate , ammonium , serum albumin , high performance liquid chromatography , biochemistry , organic chemistry , size exclusion chromatography , geography , meteorology , enzyme
The use of bovine serum albumin (BSA) in routine biochemical assays such as restriction enzyme digestion and immunodetection is plagued largely by two common contaminants, DNase and immunoglobulins G (IgGs). Acetylation of BSA
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