Open AccessIn Pursuit of Authenticity: Induced Pluripotent Stem Cell‐Derived Retinal Pigment Epithelium for Clinical Applications
Author(s) -
Miyagishima Kiyoharu J.,
Wan Qin,
Corneo Barbara,
Sharma Ruchi,
Lotfi Mostafa R.,
Boles Nathan C.,
Hua Fang,
Maminishkis Arvydas,
Zhang Congxiao,
Blenkinsop Timothy,
Khristov Vladimir,
Jha Balendu S.,
Memon Omar S.,
D’Souza Sunita,
Temple Sally,
Miller Sheldon S.,
Bharti Kapil
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2016-0037
Subject(s) - induced pluripotent stem cell , retinal pigment epithelium , biology , microbiology and biotechnology , retina , transplantation , stem cell , retinal , embryonic stem cell , neuroscience , genetics , medicine , biochemistry , gene
For effective treatment, induced pluripotent stem cell (iPSC)‐retinal pigment epithelium (RPE) must recapitulate the physiology of native human RPE cells. A set of physiologically relevant functional assays that assess the polarized functional activity and maturation state of the intact RPE monolayer is provided. The study data show that donor‐to‐donor variability exceeds the tissue‐to‐tissue variability for a given donor and provides, for the first time, criteria necessary to identify iPSC‐RPE cells most suitable for clinical application.