Open AccessRobust Differentiation of mRNA‐Reprogrammed Human Induced Pluripotent Stem Cells Toward a Retinal Lineage
Author(s) -
Sridhar Akshayalakshmi,
Ohlemacher Sarah K.,
Langer Kirstin B.,
Meyer Jason S.
Publication year - 2016
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0093
Subject(s) - reprogramming , induced pluripotent stem cell , biology , microbiology and biotechnology , cellular differentiation , stem cell , regenerative medicine , genetics , embryonic stem cell , cell , gene
The ability and efficiency of mRNA‐reprogrammed human induced pluripotent stem cells (hiPSCs) to yield retinal cell types in a directed, stepwise manner was tested. hiPSCs derived through mRNA‐based reprogramming strategies offer numerous advantages owing to the lack of genomic integration or constitutive expression of pluripotency genes. Such methods represent a promising new approach for retinal stem cell research, especially translational applications.