Open AccessEfficient Gene Editing in Pluripotent Stem Cells by Bacterial Injection of Transcription Activator‐Like Effector Nuclease Proteins
Author(s) -
Jia Jingyue,
Bai Fang,
Jin Yongxin,
Santostefano Katherine E.,
Ha Un-Hwan,
Wu Donghai,
Wu Weihui,
Terada Naohiro,
Jin Shouguang
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0030
Subject(s) - transcription activator like effector nuclease , biology , induced pluripotent stem cell , genome editing , microbiology and biotechnology , effector , crispr , green fluorescent protein , embryonic stem cell , transfection , stem cell , gene , genetics
The method of bacterial type III secretion system (T3SS)‐mediated transcription activator‐like effector nuclease (TALEN) protein injection and transfection of an oligonucleotide template to effectively generate precise genetic modifications in stem cells. T3SS‐mediated TALEN protein delivery provides a highly efficient alternative for introducing precise gene editing within pluripotent stem cells for the purpose of disease genotype‐phenotype relationship studies and cellular replacement therapies.