Open AccessA Stem Cell‐Derived Platform for Studying Single Synaptic Vesicles in Dopaminergic Synapses
Author(s) -
Gu Haigang,
Lazarenko Roman M.,
Koktysh Dmitry,
Iacovitti Lorraine,
Zhang Qi
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2015-0005
Subject(s) - dopamine , dopaminergic , neuroscience , synaptic vesicle , cellular neuroscience , biology , embryonic stem cell , vesicular monoamine transporter , microbiology and biotechnology , vesicle , monoamine neurotransmitter , biochemistry , gene , serotonin , receptor , membrane
For the use of stem cell‐derived neurons in clinical applications, improved differentiation efficiency and more careful characterization of resultant cells are needed. The authors refined a procedure for differentiation of mouse embryonic stem cells into functional dopamine neurons. This preparation provides a high yield of dopaminergic cells that are morphologically and functionally similar to cultured midbrain dopamine neurons and can be used as a platform for thorough investigation of the mechanisms of dopaminergic neurotransmission.