Open AccessEfficient Generation of Megakaryocytes From Human Induced Pluripotent Stem Cells Using Food and Drug Administration‐Approved Pharmacological Reagents
Author(s) -
Liu Yanfeng,
Wang Ying,
Gao Yongxing,
Forbes Jessica A.,
Qayyum Rehan,
Becker Lewis,
Cheng Linzhao,
Wang Zack Z.
Publication year - 2015
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0183
Subject(s) - thrombopoietin , haematopoiesis , induced pluripotent stem cell , stem cell , biology , platelet , progenitor cell , thrombopoiesis , bone marrow , microbiology and biotechnology , megakaryocyte , immunology , pharmacology , embryonic stem cell , biochemistry , gene
An efficient system was developed to generate megakaryocytes (MKs) from human induced pluripotent stem cells (hiPSCs) under a feeder‐free and xeno‐free condition, in which all animal‐derived products were eliminated. Additional maturation and investigation of hiPSC‐derived MKs should provide insights into MK biology and lead to the generation of large numbers of platelets ex vivo.