Open AccessOverexpression of Polysialylated Neural Cell Adhesion Molecule Improves the Migration Capacity of Induced Pluripotent Stem Cell‐Derived Oligodendrocyte Precursors
Author(s) -
Czepiel Marcin,
Leicher Lasse,
Becker Katja,
Boddeke Erik,
Copray Sjef
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2014-0041
Subject(s) - induced pluripotent stem cell , polysialic acid , microbiology and biotechnology , transplantation , biology , neural cell adhesion molecule , cell adhesion molecule , oligodendrocyte , cancer research , neuroscience , myelin , embryonic stem cell , cell adhesion , cell , medicine , central nervous system , biochemistry , gene
Overexpression of polysialylating enzyme sialyltransferase X (STX) was induced in iPSC‐derived oligodendrocyte precursors (OPCs) to stimulate production of polysialic acid‐neuronal cell adhesion molecules (PSA‐NCAMs), known to promote and facilitate OPC migration. STX‐overexpressing iPSC‐derived OPCs showed normal differentiation and maturation and were able to downregulate PSA‐NCAMs after becoming myelin‐forming oligodendrocytes. These findings suggest that the reach and efficacy of iPSC‐derived OPC transplantation will improve with stimulation of the OPC migration potential by specific gene modulation.