Open AccessMulticolor Staining of Globin Subtypes Reveals Impaired Globin Switching During Erythropoiesis in Human Pluripotent Stem Cells
Author(s) -
Ochi Kiyosumi,
Takayama Naoya,
Hirose Shoichi,
Nakahata Tatsutoshi,
Nakauchi Hiromitsu,
Eto Koji
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0216
Subject(s) - globin , erythropoiesis , induced pluripotent stem cell , biology , stem cell , genetics , microbiology and biotechnology , embryonic stem cell , gene , medicine , anemia
The authors examined impaired globin switching of embryonic ε‐ and fetal γ‐globin to adult β‐globin in human erythroid cells by optimizing multicolor flow cytometry to simultaneously follow expression of different globin subtypes using different immunofluorescent probes. Results showed that impaired γ‐globin silencing is associated with downregulated BCL11A‐L in human pluripotent stem cell‐derived erythroblasts and that multicolor staining of globin subtypes is an effective approach to studying globin switching in vitro.