Open AccessProneural Transcription Factor Atoh1 Drives Highly Efficient Differentiation of Human Pluripotent Stem Cells Into Dopaminergic Neurons
Author(s) -
Sagal Jonathan,
Zhan Xiping,
Xu Jinchong,
Tilghman Jessica,
Karuppagounder Senthilkumar S.,
Chen Li,
Dawson Valina L.,
Dawson Ted M.,
Laterra John,
Ying Mingyao
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0213
Subject(s) - induced pluripotent stem cell , neurodegeneration , neuroscience , biology , transcription factor , substantia nigra , dopaminergic , ectopic expression , cellular differentiation , neuron , embryonic stem cell , dopamine , disease , medicine , cell culture , genetics , gene , pathology
To fully use human pluripotent stem cells (PSCs) in regenerative medicine, highly efficient differentiation strategies are required to drive induced PSCs into desired lineages and generate functional cell progenies. Current differentiation protocols for deriving dopaminergic neurons from PSCs involve months of stem cell culture procedures and multiple reagents. This study was motivated by a desire for a rapid and highly efficient system to generate human PSC‐derived functional dopaminergic neurons.