Open AccessIncreased Genomic Integrity of an Improved Protein‐Based Mouse Induced Pluripotent Stem Cell Method Compared With Current Viral‐Induced Strategies
Author(s) -
Park Hansoo,
Kim Dohoon,
Kim Chun-Hyung,
Mills Ryan E.,
Chang Mi-Yoon,
Iskow Rebecca Cheryl,
Ko Sanghyeok,
Moon Jung-Il,
Choi Hyun Woo,
Man Yoo Paulo Sng,
Do Jeong Tae,
Han Min-Joon,
Lee Eun Gyo,
Jung Joon Ki,
Zhang Chengsheng,
Lanza Robert,
Kim Kwang-Soo
Publication year - 2014
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2013-0149
Subject(s) - klf4 , induced pluripotent stem cell , reprogramming , sox2 , biology , copy number variation , computational biology , genetics , microbiology and biotechnology , transcription factor , genome , embryonic stem cell , cell , gene
This study identified an increased copy number variant (CNV) content in lenti‐miPSCs and retro‐miPSCs (29∼53 CNVs) compared with p ‐miPSCs (9∼10 CNVs), indicating that this improved protein‐based reprogramming method maintains genomic integrity better than current viral reprogramming methods. Thus, this study demonstrates that reprogramming methods significantly influence the genomic integrity of resulting induced pluripotent stem cells.