Open AccessQuantitative Microplate Assay for Studying Mesenchymal Stromal Cell‐Induced Neuropoiesis
Author(s) -
Aizman Irina,
McGrogan Michael,
Case Casey C.
Publication year - 2013
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0119
Subject(s) - mesenchymal stem cell , nestin , microbiology and biotechnology , neurosphere , biology , embryonic stem cell , progenitor cell , stromal cell , extracellular matrix , neural stem cell , stem cell , chemistry , immunology , adult stem cell , cancer research , biochemistry , gene
This study characterizes a microplate‐format coculture system in which primary embryonic rat cortex cells were directly cocultured with human mesenchymal stromal cells (MSCs) on cell‐derived extracellular matrix in the absence of exogenous growth factors. This system enables analysis of multifactorial MSC‐neural cell interactions and can be used for elucidating the neuropoietic potency of MSCs and their derivative preparations.