Efficient Lentiviral Transduction of Human Mesenchymal Stem Cells That Preserves Proliferation and Differentiation Capabilities | Zendy

Lin Paul | Zendy; Lin Yuan | Zendy; Len Donald P. | Zendy; Correa Diego | Zendy; Schluchter Mark | Zendy; Caplan Arnold I. | Zendy

Open Access

Efficient Lentiviral Transduction of Human Mesenchymal Stem Cells That Preserves Proliferation and Differentiation Capabilities

Author(s) -

Lin Paul,

Lin Yuan,

Len Donald P.,

Correa Diego,

Schluchter Mark,

Caplan Arnold I.

Publication year - 2012

Publication title -

stem cells translational medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.781

H-Index - 71

eISSN - 2157-6580

pISSN - 2157-6564

DOI - 10.5966/sctm.2012-0086

Subject(s) - transduction (biophysics) , multiplicity of infection , mesenchymal stem cell , biology , microbiology and biotechnology , viral vector , stem cell , cell culture , fibroblast , signal transduction , genetics , gene , biochemistry , recombinant dna

This study indicates that human mesenchymal stem cells (hMSCs) can be efficiently transduced using a protamine sulfate concentration of 100 μg/ml at low volumes over 3 days. With the capacity to easily, reliably, and inexpensively transduce hMSCs, it will be possible to efficiently use genetic engineering to add or delete a function, improve or replace an existing one, and gain insight into the biology of these cells through the use of reporter genes.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research