Open AccessEfficient Lentiviral Transduction of Human Mesenchymal Stem Cells That Preserves Proliferation and Differentiation Capabilities
Author(s) -
Lin Paul,
Lin Yuan,
Len Donald P.,
Correa Diego,
Schluchter Mark,
Caplan Arnold I.
Publication year - 2012
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2012-0086
Subject(s) - transduction (biophysics) , multiplicity of infection , mesenchymal stem cell , biology , microbiology and biotechnology , viral vector , stem cell , cell culture , fibroblast , signal transduction , genetics , gene , biochemistry , recombinant dna
This study indicates that human mesenchymal stem cells (hMSCs) can be efficiently transduced using a protamine sulfate concentration of 100 μg/ml at low volumes over 3 days. With the capacity to easily, reliably, and inexpensively transduce hMSCs, it will be possible to efficiently use genetic engineering to add or delete a function, improve or replace an existing one, and gain insight into the biology of these cells through the use of reporter genes.