Open AccessOptimized Processing of Growth Factor Mobilized Peripheral Blood CD34+ Products by Counterflow Centrifugal Elutriation
Author(s) -
Tran Chy-Anh,
Torres-Coronado Monica,
Gardner Agnes,
Gu Angel,
Vu Hieu,
Rao Anitha,
Cao Lan-Feng,
Ahmed Amira,
DiGiusto David
Publication year - 2012
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.5966/sctm.2011-0062
Subject(s) - elutriation , cd34 , apheresis , progenitor cell , chemistry , chromatography , blood product , cell , plateletpheresis , blood cell , stem cell , platelet , immunology , biology , medicine , biochemistry , surgery , microbiology and biotechnology , organic chemistry
Growth factor‐mobilized peripheral blood progenitor cell products were collected from healthy donors and processed by elutriation. Fractions were collected and analyzed for cell count, viability, and blood cell differential. Using modified elutriation procedures, >99% of red blood cells and platelets were removed from apheresis products with high recoveries of total white blood cells and enrichment of CD34+ cells in one fraction. This process is proven to be a feasible method for the initial manipulation associated with primary blood cell therapy products and supports current good manufacturing process and tissue practice‐compliant cell processing.