Electrochemiluminescence Sensor for the Detection of Tiapride Hydrochloride with Chitosan-Ru(bpy)32+-SiO2NPs/MCNT/Nafion Modified Electrode

Chitosan-Ru2(bpy)32+-SiO2 composite nanoparticles (CRuS NPs) were prepared by reverse microemulsion method for the electronic attraction between negative charge of hydroxyl groups in chitosan and positive charge in Ru2(bpy)3 2+. Based on the multi-walled carbon nanotubes (MCNT)/Nafion composite membrane technology, CRuS NPs was effectively and steadily immobilized on the surface of a glassy carbon electrode. The CRuS NPs synthesized were characterized by transmission electron microscope (TEM), UV-Vis spectroscopy and fluorescence spectroscopy, while the modification was confirmed by cyclic voltammetry and electrogenerated chemiluminescence (ECL). The ECL studies revealed that the sensor had a good response to different concentrations of tiapride hydrochloride and the increased ECL intensity was directly related to the logarithm of tiapride hydrochloride concentrations in the range from 0.01 to 3.0 µmol L-1 with a detection limit of 6.0 nmol L-1. The sensor was successfully applied to the determination of tiapride hydrochloride in pharmaceutical.