Development, Optimization and Validation of an HPLC-PDA Method for Quantification of Taxifolin in the Bark Extract ofPinus pinaster | Zendy

Priscila Aparecida de Almeida | Zendy; C. A. Bhering | Zendy; Michele Campos Alves | Zendy; Marcone Augusto Leal de Oliveira | Zendy; Nádia Rezende Barbosa Raposo | Zendy; Anderson O Ferreira | Zendy; Marcos Antônio Fernandes Brandão | Zendy

Open Access

Development, Optimization and Validation of an HPLC-PDA Method for Quantification of Taxifolin in the Bark Extract ofPinus pinaster

Author(s) -

Priscila Aparecida de Almeida,

C. A. Bhering,

Michele Campos Alves,

Marcone Augusto Leal de Oliveira,

Nádia Rezende Barbosa Raposo,

Anderson O Ferreira,

Marcos Antônio Fernandes Brandão

Publication year - 2016

Publication title -

journal of the brazilian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.337

H-Index - 70

eISSN - 1678-4790

pISSN - 0103-5053

DOI - 10.5935/0103-5053.20160044

Subject(s) - taxifolin , pinus pinaster , bark (sound) , pinus <genus> , chromatography , chemistry , botany , biology , antioxidant , biochemistry , flavonoid , ecology

The aqueous extract of the bark of Pinus pinaster has a high concentration of polyphenols represented by a mixture of procyanidins, besides taxifolin, phenolic acids, cinnamic acids and their glycosides. Its quality control is specified in the United States Pharmacopeia, and the assay test is performed by determination of the total procyanidins content. However, determining the individual polyphenol content may represent an additional quality parameter for this extract. In this sense, the present study aimed to develop and optimize a method of high performance liquid chromatography with photodiode array detection (HPLC-PDA) for quantification of taxifolin in the bark extract of P. pinaster, using a 33 Box-Behnken factorial design. The proposed method was validated for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness and it has shown that taxifolin may be used as a chemical marker for quality control of the bark extract of P. pinaster.

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