Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts | Zendy

Sandro de Oliveira | Zendy; Glalci A. Souza | Zendy; Camila Rodrigues Eckert | Zendy; Thuany Alves Silva | Zendy; Edmar Silva Sobral | Zendy; Oriana A. Fávero | Zendy; Marcelo J. P. Ferreira | Zendy; Paulete Romoff | Zendy; Wilhelm J. Baader | Zendy

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Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts

Author(s) -

Sandro de Oliveira,

Glalci A. Souza,

Camila Rodrigues Eckert,

Thuany Alves Silva,

Edmar Silva Sobral,

Oriana A. Fávero,

Marcelo J. P. Ferreira,

Paulete Romoff,

Wilhelm J. Baader

Publication year - 2014

Publication title -

química nova

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.214

H-Index - 73

eISSN - 1678-7064

pISSN - 0100-4042

DOI - 10.5935/0100-4042.20140076

Subject(s) - antioxidant capacity , antioxidant , chemistry , food science , chromatography , biochemistry

The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenched per molecule of antiradical) for ascorbic acid, p-hydroquinone, catechol, quercetin, and rutin are similar when measured by colorimetric assays; however, considerably lower values of n are obtained with the luminol assay. The antiradical activity of extracts from male and female individuals of Baccharis burchelli and Baccharis crispa were determined by the luminol assay and expressed using the new Trolox® percentage (%Trolox®) parameter

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