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The aim of this study was to evaluate the effect of blood contamination on the push-out bond strength and surface morphology of tricalcium silicate materials; Biodentine  , Theracal  and mineral trioxide aggregate. The standardized lumens of root slices prepared from extracted single-root human teeth were filled with Biodentine , Theracaland mineral trioxide aggregate by manufacturer' s instruction. The specimens were randomly divided into 2 groups (n = 20) for each material and then incubated for 4 days at 37℃; control group (phosphate buffered saline solution) and experimental group (fetal bovine serum). The push-out bond strengths were then measured by a universal testing machine and the surface morphology of each experimental group was analyzed by scanning electron microscope. Biodentineand Theracalshowed higher push-out bond strength compared with mineral trioxide aggregate after exposure to fetal bovine serum. A substantial change in the surface morphology of each material was observed after exposure to fetal bovine serum. In conclusion, the push-out bond strengths of Biodentineand Theracalwere higher than mineral trioxide aggregate when exposed to blood contamination. Therefore, it is supposed that the use of Biodentine  and Theracal  is appropriate in the presence of blood. Abstract Ⅰ.

Effect of Blood Contamination on the Push-Out Bond Strength and Surface Morphology of Tricalcium Silicate Materials