Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method | Zendy

Jaehwan Kim | Zendy; Miah Kim | Zendy; Daewoo Lee | Zendy; Byeong-Ju Baik | Zendy; Yeon-Mi Yang | Zendy; JaeGon Kim | Zendy

Open Access

Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method

Author(s) -

Jaehwan Kim,

Miah Kim,

Daewoo Lee,

Byeong-Ju Baik,

Yeon-Mi Yang,

JaeGon Kim

Publication year - 2014

Publication title -

the journal of the korean academy of pedtatric dentistry

Language(s) - English

Resource type - Journals

eISSN - 2288-3819

pISSN - 1226-8496

DOI - 10.5933/jkapd.2014.41.4.292

Subject(s) - dna extraction , dna , dentistry , sonication , periodontitis , polymerase chain reaction , microbiology and biotechnology , medicine , biology , gene , chemistry , chromatography , genetics

DNA extraction is a prerequisite for the identification of pathogens in clinical samples. Commercial DNA extraction kits generally involve time-consuming and laborious multi-step procedures. In the present study, our modified DNA isolation method for saliva samples allows for the quick detection of pathogens associated with dental caries or periodontitis by PCR within 1 h. To release DNA from the bacteria, 1 min of boiling was adequate, and the resulting isolated DNA can be used many times and is suitable for long term storage of at least 13 months at 4℃, and even longer at -20℃. In conclusion, our modified DNA extraction method is simple, rapid, and cost-effective, and suitable for preparing DNA from clinical samples for PCR for the rapid detection of oral pathogens from saliva.

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