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The acute toxicity of Zn on Java medaka (Oryzias javanicus) fish was studied. To obtain the results, the experiments were carried out in 3-liter aquariums (15 to 20 fish for each aquarium) with static condition based on O.E.C.D method with 5 treatments, and 1 blank in two repetitions. During the experiment, water physico-chemical factors were pH = 7.7 to 7.9, salinity = 19.3 to 19.7 p.p.t, temperature = 29.1 to 30.8°C and conductivity = 13.54 to 13.94 ms. On the basis of obtained results, the LC50-96 h for Zn were determined at 9.75 (9.65 - 9.85) mg/l for juveniles and 14.32 (13.94 - 14.70) mg/l for adults, respectively. Also, the maximum allowable concentration (MAC) values for Zn on Oryzias javanicus juveniles and adults were determined at 0.97 mg/l for juveniles and 1.43 mg/l for adults. These results will be helpful for future ecotoxicology study based on heavy metals pollution in estuary areas.       Key words: Ecotoxicology, Oryzias javanicus, maximum allowable concentration (MAC), juveniles, adults.

Acute toxicity test of Zn, on Java medaka (Oryzias javanicus) fish as an indicator of estuary pollution