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Medicinal plants are potential sources of natural compounds with biological activities, and therefore attract the attention of researchers worldwide. The objective of this research is to determine the chemical composition of methanolic seed extract. The phytochemical compound screened by spectroscopy and gas chromatography-mass spectrometry (GC-MS) method. Sixteen bioactive phytochemical compounds were identified in the methanolic extract of Origanum vulgare. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment- ions and pharmacological actions. GC-MS analysis of O. vulgare revealed the existence of the 1,7-Dioxaspiro[5,5]undec-2-ene, 2,4-Dihydroxy-2,5-dimethyl-39(2H)-furan-3-one, 2,4-Difurobenzene, 1-benzyloxy, α-D-Glucopyranoside, O-α- Glucopyranosyl, 4-Hexenal, 6-hydroxy-4-methyl,dimethyl acetal, acetate, 4H-pyran-4-one,2,3,-dihydro-3,5-dihydroxy-6-methyl, Benzofuran, 4-Amino-1,5-pentandioic acid, 2-Methoxy-4-vinylphenol, d-Mannose, 7-Isopropyl-10-methyl-1-oxo-1,5-dithia-spiro[5,5]undecane-2-carboxy, Phytol, Cis-Vaccenic acid, N-Methyl-Nbenzyltetradecanamine, 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene-1,1,4,4-tetrone, and 17-(1,5-Dimethylhexyl)10,13-dimethyl. The FTIR analysis of O. vulgare seeds proved the presence of alkenes, aliphatic fluoro compounds, alcohols, ethers, carboxlic acids, esters and hydrogen bonded alcohols, phenols.       Key words: GC/MS, bioactive compounds, FT-IR, O. vulgare.

Biochemical analysis of Origanum vulgare seeds by fourier-transform infrared (FT-IR) spectroscopy and gas chromatography-mass spectrometry (GC-MS)