Determination of metabolites products by Penicillium expansum and evaluating antimicobial activity

The objectives of this study were to analyze the secondary metabolites of Penicillium expansum and evaluate antibacterial activity. Twenty eight bioactive compounds were identified in the methanolic extract of P. expansum. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. Gas chromatography mass spectrometry (GC/MS) analysis of P. expansum revealed the existence of the Levoglucosenone, Edulan ll, 4-[Dichloromethyl]-2-[[2-[1-methyl-2-pyrrolidinyl]ethyl]amino-6-trichloro, 1,2-Cyclopentanedione, Ethanethiol, 2-(5-(4-methyl-2-pyridyloxy)pentyl)amino-hydrogen su, Imidazole,2-amino-5-[(2-carboxy)vinyl], D-Glucose,6-O-α-D-galactopyranosyl, Eicosanoic acid , phenylmethyl ester, Dodecanoic acid, 3- hydroxyl, DL-Leucine,N-glycyl, Cyclohexene,1,5,5-trimethyl-6-acetylmethyl, 1,2-Nonadecanediol, Bicyclo[2.2.1]heptane-2-carboxylic acid  isobutyl-amide, 6-Acetyl-s-d-mannose, α-D-Glucopyranoside,O-α-D-glucopyranosyl-(1.fwdarw.3)-s-D-fruc, propanedioic acid, amino-diethyl ester, 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl, valeric acid, dodecyl ester, deoxyspergualin, I-Gala-I-ido-octonic lactone, 5-Hydroxymethylfurfural, paromomycin, 16-Nitrobicyclo[10.4.0]hexadecane-1-ol-13-one, cis-Vaccenic acid, 2-Bromotetradecanoic acid, phthalic acid,  butyl undecyl ester, picrotoxin, D-Fructose and diethyl mercaptal. The FTIR analysis of P. expansum proved the presence of aliphatic fluoro compounds, tetiary amine, C-N stretch and methylene-CH. asym which shows major peaks at 1028.06, 1151.50 and 2852.72, respectively.  Methanolic extract of bioactive compounds of P.  expansum were assayed for in vitro antibacterial activity against Proteus mirabilis, Pseudomonas aerogenosa, Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia by using the diffusion method in agar. The zones of inhibition were compared with different standard antibiotics. S. aureus has maximum zone formation (7.01±0.141) mm.   Key words: Antimicrobial activity, metabolites, bioactive compounds, GC-MS, FT-IR, P. expansum.