Evaluation of cytotoxic activity of triterpenes from Clusia studartiana | Zendy

de C. Brito Lavínia | Zendy; V. de Carvalho Marcia | Zendy; Vagner Pereira da Silva | Zendy; P. Heringer Alan | Zendy; M. da Silva Priscila | Zendy; Paula G. A. Fontao Ana | Zendy; R. Figueiredo Maria | Zendy; Luiz F. Sampaio André | Zendy

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Evaluation of cytotoxic activity of triterpenes from Clusia studartiana

Author(s) -

de C. Brito Lavínia,

V. de Carvalho Marcia,

Vagner Pereira da Silva,

P. Heringer Alan,

M. da Silva Priscila,

Paula G. A. Fontao Ana,

R. Figueiredo Maria,

Luiz F. Sampaio André

Publication year - 2019

Publication title -

journal of medicinal plants research

Language(s) - English

Resource type - Journals

ISSN - 1996-0875

DOI - 10.5897/jmpr2019.6800

Subject(s) - cytotoxicity , clusiaceae , chemistry , rhodamine 123 , phytochemical , terpene , friedelin , apoptosis , mtt assay , pharmacology , traditional medicine , stereochemistry , biochemistry , biology , multiple drug resistance , medicine , in vitro , antibiotics , triterpenoid

Family Clusiaceae is spread throughout tropical as well as temperate zones, presenting many triterpenes, reported as selective antitumoral agents. The aim of this study is to isolate and identify triterpenes from Clusia studartiana C. M. Vieira & Gomes da Silva and evaluate their cytotoxicity in melanoma (SKMEL 28) and myeloid leukemia (K562) cell lines. Thus, the hexanic extract was processed by phytochemical methods to isolate and purify the pentacyclic triterpenes: 3-oxo-friedelin (1), 3-β-hydroxy-friedelin (2) and 3-oxo-olean-12-en-28-oic-acid (3), identified by spectroscopic methods. Cytotoxicity was evaluated by MTT assay, apoptosis/necrosis by Annexin V/PI, caspase activity by FLICA in flow cytometry and P-gp modulation was measured by interference in the efflux of Rhodamine 123. From the results, triterpenes 2 and 3 showed inhibitory effect in K562 cells proliferation, and only the compound 3 was able to increase percentage of AnnexinV+PI- in cells (p<0.05), with 40% increase in caspase 3/7+ cells and showed inhibition on P-gp activity. Key words: Apoptosis, caspase, Clusiaceae, Clusia studartiana, cytotoxicity, multidrug resistance.

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