English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

A protocol for large-scale in vitro production of Baccharis myriocephala was developed. Total phenolic content was analyzed in plants cultured on MS + 1.0 mg.L-1 of indoleacetic acid (IAA), benzyladenine (BA) or kinetin (KIN). Nodal segments obtained from seeds germinated in vitro on Murashige and Skoog basal medium (MS) were subcultured on MS basal medium, hormone-free or with different growth regulators added: IAA, BA or KIN. The total phenolic content was determined using the Folin-Denis spectrophotometric method, and the content of phenolics of extracts was expressed in terms of gallic acid-equivalent (µg GA/mg dry weight). The best growth and development of the plants was induced by MS + 1.0 mg.L-1 IAA, which stimulated the production of 23 new nodal segments from a single phytomer after 60 days of in vitro culture. However, MS + 1.0 mg.L-1 IAA accumulated the lowest phenolic content, and only 18.46 µg/mg dry weight. A significant negative correlation was noted, since the phenolic content increased when the dry weight decreased. This culture system is capable of supplying good-quality raw material in adequate quantity to meet the growing demand for medicinal plants. However, the accumulation of polyphenols is lower in cultures with higher dry weight.       Key words: Carqueja, micropropagation, growth regulators, phenolics accumulation.

Effect of auxin and cytokinin on phenolic content of Baccharis myriocephala DC. (Asteraceae) produced in vitro