Method for quality DNA isolation from different endangered Indian medicinal plants | Zendy

Ramesh Malothu | Zendy; Jogayya K Naga | Zendy; Kiran D Kranti | Zendy; K Pratyusha | Zendy

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Method for quality DNA isolation from different endangered Indian medicinal plants

Author(s) -

Ramesh Malothu,

Jogayya K Naga,

Kiran D Kranti,

K Pratyusha

Publication year - 2016

Publication title -

journal of medicinal plants research

Language(s) - English

Resource type - Journals

ISSN - 1996-0875

DOI - 10.5897/jmpr2015.5869

Subject(s) - isolation (microbiology) , medicinal plants , traditional medicine , endangered species , polyphenol , dna extraction , biology , botany , microbiology and biotechnology , medicine , biochemistry , polymerase chain reaction , ecology , habitat , gene , antioxidant

Isolation of better quality DNA from endangered Indian medicinal plants such as Rauvolfia serpentina (Sarpagandha), Rhaphidophora decursiva (Atukulatiga), Piper longum (Pippali, Pippallu), Entada pursaetha (Gilateega), and Zanthoxylum Rhesta (Rhesta manu) is very much needed to further genomic study, because these are having rich source of secondary metabolites like cellulose, polyphenolic compounds, tannins, and other metabolites. The presence of certain metabolites can hamper the DNA isolation. In this study we are describing the finest method for isolation of superior quality DNA from selected medicinal plant materials by changing the composition of extraction buffer.

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