In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)
Author(s) -
Kundan Kishore,
Arifullah Mohammed,
Gopal Ghanta Rama
Publication year - 2014
Publication title -
journal of medicinal plants research
Language(s) - English
Resource type - Journals
ISSN - 1996-0875
DOI - 10.5897/jmpr2014.5440
Subject(s) - callus , plantlet , kinetin , cotyledon , explant culture , shoot , botany , seedling , biology , organogenesis , murashige and skoog medium , micropropagation , horticulture , in vitro , biochemistry , gene
were screened for their callus induction and morphogenic potential by culturing on Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), indole3acetic acid (IAA) alone or in combination with benzyladenine (BA), kinetin (KN), 2-iP (2-isopentynyl adenine), coconut milk. Multiple shoots were regenerated from cotyledonary node, shoot tip explants derived callus by one stage process where caulogenesis occurred on the primary callus. However cotyledons, leaves and root segments gave rise to adventitious shoots from callus by two stage method of indirect organogenesis. High frequency of shoot differentiation was observed from cotyledon and leaf on MS medium fortified with BA (8.9 to 13.3
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