Time and dosage effects of an endophytic fungal elicitor on the volatile oil production and physiology of Atractylodes lancea suspension cells
Author(s) -
Gang Ren,
Chen Yan,
Fang Fang,
Dai Chuan chao
Publication year - 2012
Publication title -
journal of medicinal plants research
Language(s) - English
Resource type - Journals
ISSN - 1996-0875
DOI - 10.5897/jmpr12.559
Subject(s) - elicitor , phenylalanine ammonia lyase , plant use of endophytic fungi in defense , chemistry , food science , secondary metabolite , phenylpropanoid , botany , peroxidase , biology , biochemistry , enzyme , biosynthesis , gene
-1 . The highest amount of volatile oil was harvested 9 days (d) after elicitor treatment. The activity of 3-hydroxy-3-methylglutaryl CoA reductase increased by 2.79-folds compared with the control of 7 d after treatment. The elicitor also enhanced the activities of phenylalanine ammonia-lyase, peroxidase superoxide dismutase, polyphenol oxidase, and catalase. These enhanced activities led to improved plant defence-related secondary metabolite synthesis ability. In addition, the elicitor-treated cells were consistently healthy, and had slightly increased biomass. The results demonstrated for the first time the optimal induction time and concentration of the endophytic fungal elicitor, as well as the changes in physiological indices after endophyte-host interaction. The information provided in the present study may be applied in large-scale medicinal plant suspension cultures.
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