Antimicrobial activity of the leaf extract and fractions of Lupinus arboreus | Zendy

S. C. Ohadoma | Zendy; I. O. Nnatuanya | Zendy; L. U. Amazu | Zendy; Chris E. Okolo | Zendy

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Antimicrobial activity of the leaf extract and fractions of Lupinus arboreus

Author(s) -

S. C. Ohadoma,

I. O. Nnatuanya,

L. U. Amazu,

Chris E. Okolo

Publication year - 2014

Publication title -

journal of medicinal plants research

Language(s) - English

Resource type - Journals

ISSN - 1996-0875

DOI - 10.5897/jmpr12.1263

Subject(s) - ethyl acetate , antimicrobial , aspergillus niger , maceration (sewage) , chemistry , candida albicans , fraction (chemistry) , agar , traditional medicine , agar diffusion test , food science , botany , biology , chromatography , bacteria , biochemistry , organic chemistry , medicine , materials science , genetics , escherichia coli , gene , composite material

The antimicrobial effect of the leaf extract and fractions of Lupinus arboreus was investigated. The crude methanol extract (CME) of the dried leaves obtained by 48 h cold maceration was fractionated into n-hexane fraction (HEF), ethyl acetate fraction (EAF), and methanol fraction (MEF); and evaluated using modified agar-well diffusion method. The results showed that the extract and fractions at varying concentrations, exerted strong antimicrobial activity on some of the test organisms. However, a weak activity was observed on the tested fungi-Aspergillus niger and Candida albicans. Ethyl acetate fraction showed the highest activity on many organisms than extract and other fractions. Key words: Lupinus arboreus, antimicrobial activity, standard drugs, test organisms, extracts and fractions.

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