Preliminary phytochemical screening of Quercus infectoria Oliv. for treatment of skin diseases

In present work, fruits of Quercus infectoria are selected on the basis of traditional claim. Fingerprinting analysis for the said extract along with the biomarker, gallic acid was performed using high performance thin layer chromatography (HPTLC). The extract and biomarker were standardized with various instrumental techniques like Newton magnetic resonance (NMR), Fourier transform infrared (FTIR) and ultraviolet (UV) spectroscopies. Preliminary phytochemical screening was performed for establishing the profile of extract for its nature of chemical composition. The extracts showed the presence of tannins, mucilage and saponins. HPTLC fingerprinting showed better separation of the components. Planer chromatogram generated was used to determine existence of present phytoconstituents. The Rf value was found to be 0.19. The extract exhibited good correlation with selected marker in different analytical techniques like NMR, FTIR and UV spectroscopies. UV-Spectroscopic analysis showed good correlation between plant extract and standard (gallic acid). The extract and biomarker exhibited 0.074, 0.0511 and 0.003, 0.0429 intercept and slope values, respectively.   Key words: Quercus infectoria, gallic acid, high performance thin layer chromatography (HPTLC), Newton magnetic resonance (NMR), Fourier transform infrared (FTIR), ultraviolet (UV) spectroscopy, tannins.