The effect of cytokinins on in vitro shoot length and multiplication of Hymenocallis littoralis

This study was performed to determine the effects of different cytokinins at various concentrations on the in vitro shoot formation of Hymenocallis littoralis. The bulb scales of H. littoralis were used as explants to establish the cultures. The explants were grown on semi-solid Murashige and Skoog (MS) medium supplemented with 2iP (2-isopentenyladenine), TDZ (thidiazuron) and Zeatin respectively at six different concentrations (2.25, 4.50, 9.00, 13.50, 18.00, and 22.50 μM). Two subcultures were performed at 30 days interval after the initial in vitro culture establishments. It was found that 2iP and concentration 13.50 μM respectively was the best for shoot elongation which was measured in length (cm). However, the three cytokinins and the six tested concentrations were recorded to have no significant difference in terms of shoot multiplication. Highest total chlorophyll content was observed in shoots grown on semi-solid MS medium supplemented with 2.25 μM of Zeatin.