Acaricidal activity of Chrozophora oblongifolia on the two spotted spider mite, Tetranychus urticae Koch

The acaricidal activity of Chrozophora oblongifolia (Delile) Spreng. (Euphorbiaceae) extracts collected from Dakhla Oasis, Western Desert of Egypt was examined against larvae and adult females of Tetranychus urticae Koch (Acari: Tetranychidae). Acaricidal activity-guided isolation of methylene chloride, ethyl acetate, butanol fractions resulted in separation and identification of p-hydroxybenzaldehyde (1), 3,5-dimethoxy-4-hydroxybenzaldehyde (2), scopoletin (3), amentoflavone (4), apigenin 7-O-β-D- glucopyranoside (5) and apigenin 7-O-β-D-[2",6"-bis(4-hydroxy-E-cinnamoyl)] glucopyranoside (6). The isolated compounds were identified by MS and NMR spectral analyses. The susceptibility of the larvae and adult females of T. urticae Koch to the tested isolated compounds revealed that apigenin 7-O-β-D-[2",6"- bis(4-hydroxy-E-cinnamoyl)] glucopyranoside (6) and  apigenin 7-O-s-D- glucopyranoside (5) isolated from butanol fraction (most effective fraction) exhibited a high degree of acaricidal activity using leaf-dipping technique against larvae after 7- days of exposure, respectively. Key words: Acaricidal activity, phytochemistry, Tetranychus urticae Koch, Chrozophora oblongifolia, Euphorbiaceae.