Algal density assessed by spectrophotometry: A calibration curve for the unicellular algae Pseudokirchneriella subcapitata | Zendy

Helena Ribeiro Rodrigues Lúcia | Zendy; Arenzon Alexandre | Zendy; Teresa Raya-Rodriguez Maria | Zendy; Ferreira Fontoura Nelson | Zendy

Open Access

Algal density assessed by spectrophotometry: A calibration curve for the unicellular algae Pseudokirchneriella subcapitata

Author(s) -

Helena Ribeiro Rodrigues Lúcia,

Arenzon Alexandre,

Teresa Raya-Rodriguez Maria,

Ferreira Fontoura Nelson

Publication year - 2011

Publication title -

journal of environmental chemistry and ecotoxicology

Language(s) - English

Resource type - Journals

ISSN - 2141-226X

DOI - 10.5897/jece2011.025

Subject(s) - absorbance , calibration curve , algae , spectrophotometry , optical density , environmental chemistry , chemistry , chromatography , botany , physics , biology , detection limit , optics

The unicellular algae Pseudokirchneriella subcapitata (Korschikov) Hindak has been frequently used for ecotoxicological tests. In this paper, a calibration curve is proposed correlating absorbance values (684 nm) and cell density for routine ecotoxicological experiments. Density (cells/mL) could be estimated as follow: Cell Density = e

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