A study on the formation and development of Panax bipinnatifidus Seem. adventitious root
Author(s) -
Bùi Đình Thạch,
Tu Linh Lenguyen,
ThiThuy Van Nguyen,
Thi Ben Trinh,
Truong Mai,
Huu Ho Nguyen
Publication year - 2014
Publication title -
journal of developmental biology and tissue engineering
Language(s) - English
Resource type - Journals
ISSN - 2141-2251
DOI - 10.5897/jdbte2014.0069
Subject(s) - callus , sucrose , botany , chemistry , biology , food science
This study aimed to generate adventitious roots from root and stem-derived callus of Panax bipinnatifidus Seem. Callus formation was observed best (83.3%) in MS media supplemented with 3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg/L indolebutyric acid (IBA). Callus generated adventitious roots in MS media supplemented with 2.0 mg/L IBA at high frequency (66.7%). The optimal culture condition for the development of adventitious roots was liquid MS media supplemented with 40 mg/L sucrose and maintained at pH 6.0. Key words: Adventitious root, stem, basic MS media.
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